Figure 6.

Concentration-dependent effects of TGF-β (A) and effects of 0.03 ng·mL⁻¹ TGF-β in absence and presence of bosentan (Bos, 10 μM) (B) on release of ET-1 by MRC-5 human lung fibroblasts. After dissemination, cells were cultured for 24 h in presence of 10% FCS followed by 48 h in presence of 1% FCS and absence or presence of test substances as indicated. The last medium change was performed 6 h before the supernatant were collected for ET-1 determination by elisa. ET-1 accumulation is expressed as % of the control of the individual cell preparation, given are means ± SEM of n ≥ 12. Significance of differences: **P < 0.01; ***P < 0.001 versus Ctr.; ⁺P < 0.05 versus respective value in absence of Bos.