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Time- and concentration-dependent conversion of big-ET-1 into ET-1 by MRC-5 human lung fibroblasts in culture. Big-ET-1 at the concentrations given was added to nearly confluent cultures in absence of FCS for 1 to 8 h, as indicated, followed by determination of ET-1 accumulation in the supernatant. Given are means of n ≥ 6, for clarity of the figure without SEM, which were between 5% and 15% of the respective mean value.
