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. 2012 Dec 20;168(2):471–487. doi: 10.1111/j.1476-5381.2012.02190.x

Figure 8.

Figure 8

Effects of TGF-β on expression of ET-A and ET-B receptors in MRC-5 and primary (phLFb) human lung fibroblasts. After dissemination cells were cultured for 24 h in presence of 10% FCS followed by 24, 72 h or 7 days in presence of 1% FCS and absence or presence of TGF-β at the concentrations indicated. Thereafter, total RNA was isolated, treated with DNase and used for quantitative real time PCR. Height of columns: ET-A or ET-B mRNA (ΔCt over GAPDH) is expressed as % of the control of the individual cell preparation, given are means ± SEM of n ≥ 3. Significance of differences: ***P < 0.001; **P < 0.01; *P < 0.05 versus respective controls.