Figure 13.
Effect of ET-1 in absence or presence of bosentan (Bos) on collagen I-α1 mRNA expression in MRC-5 human lung fibroblasts; 105 cells were seeded in 12-well dishes and first cultured for 24 h in presence of 10% FCS, followed by additional 24 h under FCS-free conditions in absence or presence of test substances at the concentrations given. Thereafter, total RNA was isolated, treated with DNase and used for quantitative real time PCR. Height of columns: collagen I-α1 mRNA (ΔCt over GAPDH) is expressed as % of the control of the individual cell preparation, given are means ± SEM of n ≥ 6. Significance of differences: ***P < 0.01 versus control (Ctr.); +++P < 0.001 versus respective value in absence of Bos.