Figure 14.

Effects of TGF-β and of bosentan (Bos, 10 μM) in absence and presence of TGF-β on [³H]-proline incorporation in MRC-5 (A); 10⁵ cells were seeded in 12-well dishes and first cultured for 24 h in presence of 10% FCS, followed by additional 24 h under FCS-free conditions. Thereafter, [³H]-proline (37 kBq) was added alone or in combination with test drugs, and cells were cultured for further 24 h. Radioactivity incorporated in cellular and extracellular protein was determined and is expressed as % of the mean value of the respective controls (in absence or presence of TGF-β) of each cell preparation. Given are means ± SEM of n ≥ 8 experiments. Significance of differences: ***P < 0.001 versus respective Ctr.; ⁺P < 0.05 versus respective value in absence of TGF-β.