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SDS–PAGE and western blotting analyses of purified ERB–HP-DDADD-RNase. (A) SDS–PAGE analysis followed by Coomassie staining of the samples eluted by IMAC. Lane 1: Molecular weight standards. Lane 2: fractions eluted from the column. (B) Western blotting analysis with an anti-His antibody. Lane 3: fractions eluted from the column.
