Table 3.
Targets from shikimate pathway: relationship between MIC, flux, and the type of inhibition
| Gene |
Concentration for MIC
|
|||||
|---|---|---|---|---|---|---|
| Competitive | Noncompetitive | Uncompetitive | ||||
|
|
|
|
||||
| Drug concentration (μM) | Flux (μmol/second) | Drug concentration (μM) | Flux (μmol/second) | Drug concentration (μM) | Flux (μmol/second) | |
| Control | 0 | 0.147 | 0 | 0.147 | 0 | 0.147 |
| aroA | 80,000 | 0.0002 | 30,000 | 0.0019 | 500 | 5E-04 |
| aroB | 120,000 | 0.0539 | 5000 | 0.0024 | 1000 | 0.012 |
| aroC | 80,000 | 0.0598 | 5000 | 0.0008 | 250 | 0.015 |
| aroD | 400,000 | 0.0522 | 5000 | 0.0043 | 3000 | 0.007 |
Note: The concentrations that brought in growth arrest (MIC) were different amongst the various targets, inhibitor type, and flux through the pathway.
Abbreviation: MIC, minimum inhibitory concentration.