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Siamois/Twin occupancy at organizer promoters is enhanced by Nodal signaling. Genomic regions recovered by ChIP for 10 pg myc-Sia, 10 pg myc-Sia and 50 pg Xnr1, 10 pg myc-Twn, or 10 pg myc-Twn and 50 pg Xnr1 were evaluated by QPCR for the (A) Gsc, (B) Cer or (C) Chd promoters. The white bars represent QPCR for genomic EF1α as a control. The mean fold enrichment (normalized to uninjected samples) and standard error for eight independent experiments is presented.
