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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Cell. 2011 Feb 18;144(4):513–525. doi: 10.1016/j.cell.2011.01.020

Figure 6. Dpy-30 Is Important for Normal H3K4me3 Increases at Developmental Genes for RA-Mediated Cell-Fate Transitions.

(A) H3K4me3 levels at the representative developmental genes and an intergenic region in control (S), RbBP5- (R), or Dpy-30-depleted (D) cells before and after RA-mediated differentiation in monolayer culture. In A-D, signals were determined by ChIP-qPCR. InA and D, averages ± SD from triplicate reactions are plotted.

(B) H3K4me3 levels at a large panel of genomic regions after RA-mediated differentiation in monolayer culture. Note the gene list is exactly the same as that in Figure 2E, and a general increase of H3K4me3 is apparent in the comparison to Figure 2E. Averages ± SD from duplicate reactions are plotted.

(C) H3K4me3 levels at the broad TSS-proximal regions of HoxC5, HoxC6 and Punc genes before and after RA-mediated differentiation in monolayer culture.

(D) Dpy-30 (left) and RNA Pol II (right) recruitment at some RA-inducible genes and an intergenic region before and after RA-mediated differentiation in monolayer culture.

See also Figure S5.

Figure 6