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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Parasite Immunol. 2012 Oct;34(10):464–472. doi: 10.1111/j.1365-3024.2012.01380.x

Figure 1. IDO is Induced in Human DC in Response to Leishmania Infection.

Figure 1

A)IDO mRNA transcript expression as measured by qRT-PCR using equal concentrations of cDNA from six healthy adult human monocyte-derived DC and normalized to HPRT. Cells were left uninfected (UI) or infected in vitro with L. major (Lm) or L. donovani (Ld) or exposed to 1μg/mL of LPS (LPS) for 16 hrs. B) Western blot analysis of human monocyte-derived DC infected with L. major, L. donovani, or stimulated with LPS (1μg/mL) for 48 hrs. Blots were probed for IDO and GAPDH as a loading control. Data from one of six representative donors is presented. C) qRT-PCR of IDO mRNA from human monocyte-derived DC, infected in vitro with live L. major or stimulated with an equivalent amount of parasite lysate for 16 hrs. D) IDO mRNA detected from uninfected or L. major infected DC from the lower chamber of a transwell system. Infection in upper wells as indicated, n = nine donors. Data is expressed as mean ± standard error of th mean (SEM) fold induction over uninfected controls, *p ≤ 0.05 by Mann-Whitney Test.