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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Neuron. 2004 Oct 28;44(3):469–482. doi: 10.1016/j.neuron.2004.10.010

Figure 6. High Cl− Inhibits Endocytosis in Dissociated Terminals under 5 mM Internal EGTA.

Figure 6

(A) At 0.5 mM EGTA, Ca2+-activated chloride tail currents [ICl(Ca); arrows] are present under both high- and low-Cl− internal solutions. In contrast, ICl(Ca) is completely blocked with both high- and low-chloride internals in 5 mM EGTA.

(B) Membrane capacitance was averaged for six terminals with high chloride (blue) and eight terminals with low chloride (red) under 5 mM EGTA. In high chloride, the averaged endocytosis was best fit with a single, slow exponential. Averaged endocytosis was significantly faster under low chloride and was best fit with a single exponential.

(C and D) The amplitude of ICa++ was the same for high and low Cl− under 5 mM EGTA, and exocytosis was reduced by ~73% as compared to 0.5 mM EGTA.