TABLE 1.
Day- and nighttime recordings in nystatin perforated patch mode
| Depolarization | ΔCm, fF | Peak ICa2+, pA | QCa2+, pC | n |
|---|---|---|---|---|
| 2 ms | ||||
| Day | 30.2 ± 3.2 | 172.9 ± 10.4 | 0.32 ± 0.02 | 13 |
| Night | 22.7 ± 4.1 | 265.8 ± 34.8* | 0.41 ± 0.05 | 12 |
| P value | 0.1593 | 0.0144 | 0.0986 | |
| 20 ms | ||||
| Day | 50.1 ± 4.8 | 189.9 ± 8.1 | 3.81 ± 0.87 | 21 |
| Night | 27.3 ± 3.1** | 276.4 ± 18.4* | 5.42 ± 0.37 | 15 |
| P value | 0.0003 | <0.0001 | 0.1450 | |
| 200 ms | ||||
| Day | 80.2 ± 10.0 | 218.8 ± 16.8 | 41.61 ± 3.09 | 23 |
| Night | 87.1 ± 13.0 | 304.8 ± 34.1* | 55.19 ± 6.08* | 13 |
| p value | 0.6786 | 0.0159 | 0.0334 |
Averaged exocytosis (ΔCm) peak calcium current (ICa2+) calcium charge (QCa2+ integral of the calcium current), and the number of terminals (n) recorded with nystatin perforated-patch mode during day- and nighttime. Voltage-clamp depolarizations were elicited from a holding potential of −60 to 0 mV for 2, 20, or 200 ms. For each depolarization, the peak calcium current was significantly larger for the set of terminals recorded at night (*P < 0.05). For 2- and 20-ms depolarizations, the average ΔCm was larger during the day than at night although this difference was only significant at 20 ms (**P = 0.003). Averages have been rounded to the nearest significant digit.