FIGURE 2.
Examples of two-parameter flow cytometry dot plots, showing the simultaneous binding of Annexin V and 7-AAD uptake by cultured rat astrocytes after the induction of necroptosis. For each treatment, ten samples from two independent groups of animals were analysed. (A) Cells were not exposed to staurosporine (controls). (B) Cells were exposed to 10−6 M staurosporine for 3 hours, and regenerated for 22 hours in a staurosporine free medium. (C) Cells were exposed to 10−6 M staurosporine for 3 hours, and analysed without regeneration.