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. 2012 Nov 9;46(4):312–320. doi: 10.2478/v10019-012-0036-9

FIGURE 3.

FIGURE 3.

Percentages of a cell death in cultured rat astrocytes after the exposure to 10−7 M staurosporine. Early apoptosis and necroptosis were determined by the binding of Annexin V-FITC and 7-AAD uptake, using a flow cytometry. Data are the means ± SEM of ten samples from two independent groups of animals. For the z-vad-fmk treatment, only five samples were analysed. (Control) Control cells were not exposed to staurosporine or z-vad-fmk. (STS) Cells were exposed to 10−7 M staurosporine for 6 hours, and regenerated for 22 hours in a staurosporine free medium. (STS+z-vad-fmk) Cells were exposed to staurosporine and 4 x 10−5 M z-vad-fmk for 6 hours, and regenerated for 22 hours in a staurosporine free medium with z-vad-fmk. (z-vad-fmk) Cells were exposed to 4 x 10−5 M z-vadfmk for 24 hours. Data were analysed using the non-parametric Mann-Whitney U test; * p < 0.00 vs Control-early apoptosis, ** p < 0.00 vs STS-early apoptosis and *** p = 0.002 vs STS-necroptosis indicate significance.