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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Biochemistry. 2013 Jan 11;52(3):466–476. doi: 10.1021/bi301341r

Figure 1.

Figure 1

A time/aging dependent influence on the affinity of L-PYK for PEP. A) The initial velocity derived Kapp-PEP for wild type L-PYK as a function of storage time after purification (36 hrs. after cell lysis are required for purification). The line represents the data trend. B) The influence of “aging” on allosteric properties elicited by Fru-1,6-BP (squares) and by alanine (circles). The influence of ageing appears to primarily impact PEP affinity since the allosteric properties (the distance between the plateaus at low and high effector concentrations) is relatively unchanged for freshly purified (solid symbols) vs. “aged” (open symbols) enzyme. Lines represent the best fits to Equation 1.