. Author manuscript; available in PMC: 2013 Aug 1.

Published in final edited form as: Protein Expr Purif. 2012 Jun 19;84(2):224–235. doi: 10.1016/j.pep.2012.06.005

Table 5.

Description of peptides explored in this work. Forward primer (FP) and reverse primers (RP) listed in Table 5.

GPCR Peptide	AA	Molecular weight^a	Oligonucleotides	PCR Template
hA₂aR TM 7	259–316	6962.198	FP A₂aR 259, RP A₂aR 316	pCDNA 3.1 hA₂aR
hA₂aR TM 6–7	205–316	13152.621	FP A₂aR 205, RP A₂aR 316	pCDNA 3.1 hA₂aR
hA₂aR TM 5–7	170–316	17435.893	FP A₂aR 170, RP A₂aR 316	pCDNA 3.1 hA₂aR
hA₂aR TM 6	205–265	6922.217	FP A₂aR 205, RP A₂aR 265	pCDNA 3.1 hA₂aR
Rant 5 TM 6–7	205–316	12968.379	FP A₂aR 205, RP A₂aR 316	pRG/III-hs-MBP-Rant 5 [65]
hA₁R TM 7	260–326	7815.028	FP A₁R 260, RP A₁R 326	pCDNA 3.1 hA₁R
hA₁R TM 6–7	208–326	13717.173	FP A₁R 208, RP A₁R 326	pCDNA 3.1 hA₁R
hA₂bR TM 7	260–332	8087.404	FP A₂bR 260, RP A₂bR 332	pCDNA 3.1 hA₂bR
hA₂bR TM 6–7	211–332	13689.100	FP A₂bR 211, RP A₂bR 332	pCDNA 3.1 hA₂bR

Molecular weights are based on the theoretical molecular weights calculated from the peptide primary sequence [70] and correspond to the peptide alone without contributions from the expression cassette.