Figure 3.

Dot blot analysis of α-synuclein fibrils in striatal urea extracts of human BAC synuclein transgenic rats. Dot-blot analysis was performed using FILA-1 antibody specific for aggregated α-synuclein and an antibody for human α-synuclein (huSyn) to confirm its aggregation. The levels of FILA-1 signals in individual rat samples were normalized to the average total levels of α-synuclein (human + rodent α-synuclein). The levels of human α-synuclein are expressed relative to the background signals detected in wild-type control rats. Values are represented as mean + SEM. The FILA-1 antibody detected specifically fibrillized recombinant α-synuclein (rSYN) of the standard and in striatal urea extracts (Urea S) of aged transgenic (Syn) rat brain. Both FILA-1 and human α-synuclein antibodies detected an average doubling of insoluble α-synuclein aggregates at 16 month when compared with 3-month-old rats (*P < 0.05; two-tailed t-test).