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. 2013 Feb 14;8(2):e56591. doi: 10.1371/journal.pone.0056591

Figure 5. Expression of MMP-2, uPA and PAI-1 in HBCEC, HMEC, and permanent breast cancer cell lines.

Figure 5

a, QPCR determination of MMP-2 expression in MDA-MB-231, MDA-MB-435s, and MCF-7 cells (upper graph) and in HMEC and various HBCEC (lower graph) after stimulation with TGF-β1 (10 ng/ml) for 24 h and 48 h. Data are presented as mean ± SD of three reactions after normalization with the housekeeping genes TBP and β-actin. The numbers above the graphs indicate -fold induction by TGF-β1 relative to unstimulated controls. Asterisks indicate significant differences relative to the respective treatment groups in HMEC. b, Expression of uPA (upper graphs) and PAI-1 (lower graphs) in MDA-MB-231 and MCF-7 cells (left graphs), and HMEC and HBCEC (right graphs) as measured by ELISA. Data represent the mean ± SD of duplicate determinations. (n = 2 to 3). n.d., not detectable.