Table 1. The statics of the translational movements of the KIF1A head in one ATP-hydrolysis cycle.
#sample | No-detach | +8 nm | 0 nm | −8 nm | ?2 * | P-value** | |
stand-alone/strong | 100 | 99 | 0 | 0 | 1 | 1.00 | 3.17×10−1/Accepted |
stand-alone/weak | 235 | 49 | 44 | 92 | 50 | 0.383 | 5.36×10−1/Accepted |
Cargo/strong | 109 | 0 | 52 | 56 | 1 | 49.1 | 2.46×10−12/Rejected |
cargo/weak | 150 | 0 | 43 | 81 | 26 | 4.19 | 4.07×10−2/Rejected |
Stand-alone/weak/DH | 80 | 6 | 12 | 46 | 16 | 0.571 | 4.50×10−1/Accepted |
) The chi-square test for the null hypothesis “If the KIF1A head succeeds in detaching itself from MT, the forward and backward binding events are equally probable.”
Based on the null hypothesis, the probability to observe the current data. The null hypothesis was “accepted” or “rejected” by the significance level 0.05. Data here do not include re-detachment and re-attachment processes, which did not produce any significant bias (see Fig. 9 and sub-section “The statics of the re-detachment and the reattachment process”).