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. 2013 Feb 14;8(2):e56274. doi: 10.1371/journal.pone.0056274

Figure 2. The headbobber mutation.

Figure 2

A: Plot showing the main genomic variations found by aCGH in 4 hb/hb on Chromosome 7: a 647.7 kb deletion in qF3, confirmed by deep sequencing (red arrow, loss of copy number, Chr7: 139061190–139708657, log ratio of −8.5) and a gain in qF3 (blue arrow, Chr7: 138832773–138832773, log ratio 1.3). B: Screenshot of the deleted region (www.ensembl.org). The red square delimits the breakpoints of the deletion. There are three protein coding genes in the region (Gpr26, Cpxm2 and Chst15), a non-coding RNA (n-R5s159) and an antisense RNA (Gm15677). C: Quantitative real-time PCR on cDNA generated from RNA from P1 cochleae show the absence of mRNA for Gpr26, Cpxm2 and Chst15 in hb/hb compared to the littermate controls. Error bars, s.d. Quantity normalised to Hprt1 levels. N = 3.**: p<0.01.