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. 2013 Feb 14;8(2):e56308. doi: 10.1371/journal.pone.0056308

Figure 5. Inducible expression of EVI1 decreases the sensitivity of HL-60 cells to daunorubicin.

Figure 5

HL-60_EVI1 and HL-60_vec cells were incubated with 2 µg/ml doxycycline for 24 h prior to addition of daunorubicin (DR) at the indicated concentrations for another 48 h. A) Metabolic activity was measured based on ATP content. B) The proportions of viable, apoptotic, and dead cells were determined via the annexinV (AV)/propidium iodide (PI) assay. Double negative cells were considered viable, PI- AV+ cells apoptotic, and PI+ cells dead. C) Proportion of eGFP positive (eGFP+) cells in HL-60_EVI1 and HL-60_vec cells treated with various doses of daunorubicin. Data points in all panels represent the mean +/− standard deviation (SD) from three independent biological replicate experiments. *, p<0.05; ***, p<0.001 (paired Student’s t-test).