Table 1. Primers used for amplification and sequence analysis of the integrated, vector borne EVI1 gene from U937_EVI1 cells.
Primer name | sequence (5′–3′) |
F1-F* | GCTTGGATACACGCCGC |
F1-R | GGTCACTGCCACTTGGTGTA |
F2-F | GAGGTTTTGTGAGGGCAAGA |
F2-R | ACCCACTCCTTTCTTTATGGACC |
F3-F | ACCCACACAGGAGAGCAGCC |
F3-R | TACGTGGCTTATGGACTGGATAGCAC |
F4-F | AGCAACGTCGAATCAAGACCTGCTTCAGAT |
F4-R | CGGACTAGTTGCTGTACCGGACATGTTCCC |
F5-F | TGCTCTAGATCATATAAGGCA |
F5-R* | GGAGAGGGGCGGAATTTAC |
Primers F1-F and F5-R match the vector, rather than the EVI1, sequence.