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. 2013 Feb 14;8(2):e56308. doi: 10.1371/journal.pone.0056308

Table 1. Primers used for amplification and sequence analysis of the integrated, vector borne EVI1 gene from U937_EVI1 cells.

Primer name sequence (5′–3′)
F1-F* GCTTGGATACACGCCGC
F1-R GGTCACTGCCACTTGGTGTA
F2-F GAGGTTTTGTGAGGGCAAGA
F2-R ACCCACTCCTTTCTTTATGGACC
F3-F ACCCACACAGGAGAGCAGCC
F3-R TACGTGGCTTATGGACTGGATAGCAC
F4-F AGCAACGTCGAATCAAGACCTGCTTCAGAT
F4-R CGGACTAGTTGCTGTACCGGACATGTTCCC
F5-F TGCTCTAGATCATATAAGGCA
F5-R* GGAGAGGGGCGGAATTTAC
*

Primers F1-F and F5-R match the vector, rather than the EVI1, sequence.