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. 2013 Feb 14;8(2):e56666. doi: 10.1371/journal.pone.0056666

Figure 4. Berberine induces EGFR down-regulation and inhibits proliferation in colonic epithelial cells in APC min/+ mice.

Figure 4

5–6 week old mice were gavaged with berberine at 100 mg/kg bodyweight for 3 days with or without EGF injection for 2 hours before sacrificed. (A–B) Colonic epithelial cells were collected for Western blot analysis to detect the indicated signaling pathways. The protein band ratio is calculated by comparing the relative density of the protein band on Western blots to that of β-actin band from the same mouse. The average ratio in control was set as 100%, the fold change of the ratio in treated mice is shown. (C–D) colon tissue was fixed and prepared for immunohistochemistry of ki67. Brown nuclei represent ki67 positive staining. Ki67 positive staining cells were counted in at least 100 crypts. The number of ki67 positive staining cells per crypt is shown. N = 5 mice in each treatment group.