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. 2012 Nov 19;7(2):359–364. doi: 10.3892/mmr.2012.1189

Figure 1.

Figure 1

Establishment of core2 β-1,6-N-acetylglucosaminyltransferase (C2GnT)-deficient prostate cancer cells. (A) Biosynthesis pathway of O-glycan core structures, core1-4. N-acetylgalactosamine (GalNAc) is transferred to serine (Ser) or threonine (Thr) residues in a polypeptide by peptide GalNAc transferase (GalNAc-T). GalNAcα1-Ser/Thr is converted by Core1 synthase to Galβ1-3GalNAcα1-Ser/Thr (core1). Core1 is then converted by C2GnT-1 to core2. Core1 is also converted by Core3 synthase to core3. Core3 is converted by C2GnT-2 to core4. β-1,4-galactosyltransferase IV (β1-4Gal-T IV) together with β-1,3-N-acetylglucosaminyltransferase (β1-3GlcNAc-T) synthesize poly-N-acetyllactosamine in core2 branched oligosaccharides. Lycoperiscon esculentum (tomato) lectin (LEL) binds specifically to poly-N-acetyllactosamine with at least three lactosamine unit repeats. (B) Relative expression levels of C2GnT in prostate cancer cell lines were analyzed by RT-PCR. (C) Cell morphologies of PC3 and C2GnT-deficient cells. Bar is 50 μm.