Figure 6. Decrease of Rab28 expression attenuated activation of NF-κB in ECs.

(A) As a control, ECs were incubated with Lipofectamine 2000 and negative control siRNA. Then cells were stimulated with 10⁻⁶ mol/L Ang II for 30 minutes. Fluorescence microscopy showed the translocation of NF-κB from cytoplasm into nucleus (activation) was significant. (B) ECs were incubated with Lipofectamine 2000 and target siRNA of Rab28. Then cells were stimulated with 10⁻⁶ mol/L Ang II for 30 minutes. Fluorescence microscopy showed the attenuated Rab28 expression and NF-κB translocation. (A) and (B) were photographed with the same exposure time. Scale bars: 10 µm. (C) siRNA effectively knocked down the expression of Rab28. (D) After the knockdown of Rab28, the phosphorylation of NF-κB p65 (activation) were significantly attenuated. All results are given as mean ± s.d., **P<0.01, n = 7 each.