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. 2013 Feb 14;8(2):e54867. doi: 10.1371/journal.pone.0054867

Figure 1. Hog1p phosphorylation level and growth phenotypes for the wild type (WT) and mutant yeast cells under various osmotic and salt stress conditions.

Figure 1

A. Hog1p MAPK phosphorylation (P-Hog1p) was detected in the ssk1Δste11Δ mutant under hyperosmotic stress. Cells were exposed to different level of osmotic stress induced by sorbitol (concentration shown) in YPD medium for the time indicated. B. Same experiment as in A but for the wild type strain which shows higher sensitivity and a longer duration of the response. C. Hog1p phosphorylation was not detected in the ste11Δssk2Δssk22Δ mutant. D. Hog1p phosphorylation assay under ionic osmotic stress in the ssk1Δste11Δ double mutant. Cells were exposed to a different levels of salt stress induced by NaCl (concentration shown) in YPD medium for the time indicated. E. Same as in D but for the wild type cells. F. The ssk1Δste11Δ mutant exhibited better growth than hog1Δ mutant under osmotic stress. Serial dilutions (from left to right in each panel) of indicated strains were spotted onto YPD and salt plates and growth was scored after 3 days.