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. 2013 Feb 14;8(2):e54867. doi: 10.1371/journal.pone.0054867

Figure 2. Ssk2p can be activated independent of Ssk1p under severe osmotic stress.

Figure 2

A. Hog1p was phosphorylated in the ste11Δssk1Δssk22Δ mutant under severe osmotic stress (higher than 0.5 M sorbitol). B. Hog1p could not be phosphorylated in the ste11Δssk1Δssk2Δ mutant under 0.4 M or 1.0 M sorbitol. C. Actin disassembly did not activate the HOG pathway through Ssk2p. Within Lat B treatment, wild type strain and ste11Δssk1Δ mutant did not display activation of Hog1p. D.The effect of Lat B on actin structures in yeast cells. Rd-phalloidin was used to observe the effects of Lat B addition to yeast cells. Both the wild type cells and ste11Δssk1Δ mutant cells were incubated in the absence of Lat B and for 20 min in the presence of 200 mM Lat B. E. The osmosensitivity phenotype of budding yeast HOG pathway mutants. Serial dilutions (from left to right in each panel) of indicated strains were spotted onto YPD and salt plates and growth was scored after 3 days.