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. 2013 Feb 14;7(2):e2048. doi: 10.1371/journal.pntd.0002048

Figure 1. OAg-AcrA glycoconjugate as an antigen for the diagnosis of human brucellosis.

Figure 1

A) 10% SDS-PAGE analysis of purified non-glycosylated (NG) and glycosylated AcrA (G) by Coomassie brilliant blue and immunoblot using anti-histidine tag and anti-Brucella O-antigen monoclonal antibodies. B) Glycoconjugate magnetic beads-based immunoassay for detection of antibodies against the O-polysaccharide fraction of lipopolysaccharide (LPS). Magnetic beads coated with OAg-AcrA were incubated with the indicated human serum samples. Bound antibodies were detected using Cy5-conjugated goat anti human IgM/G antibodies.Positive and negative controls; human sera included as positive and negative controls in each assay run. The figures in parenthesis correspond to the identification of the samples according to Tables S1 and S2. Results are expressed as percentage of reactivity of the positive control serum. The bar graph data represents the means and standard deviation for two separate determinations. C) Western blot analysis of the same human serum samples.