Figure 3. IgG antibody response in C57BL/6 wild-type (WT) and TLR4 KO mice after immunization with MSP-3 in the absence of adjuvant.

Groups of 5 mice were immunized 3 times (s.c.) with 10 µg of PvMSP-3α, PvMSP-3β (FP-1), PvMSP-3β (FP-2), or PvMSP-3β (FP-3) and antibody titers to homologous PvMSP-3 were determined after each dose. Results are expressed as the means of antibody titers (log₁₀) ± SEM and were compared by one-way ANOVA followed by Tukey’s test for multiple comparisons. After the third dose, non-significant differences between groups of immunized mice (C57BL/6 WT vs. TKR4 KO) are denoted on the graph as “n.s.” Significant difference between 2 groups of mice immunized with 3 doses of PvMSP-3β (FP-2) are denoted on the graph (*p<0.05).