Figure 1.

Detection of AGT, renin, ACE and AT1R mRNA expression by real-time PCR. Amplification products of (panel A) AGT (128 bp), renin (121 bp), ACE (164 bp), and (panel B) AT1R (80 bp) and GAPDH (225 bp) obtained from placental cell total RNA were visualized on ethidium bromide stained 2% agarose gels. Reverse transcriptase (RT) was present (+) or absent (-) in the step of reverse transcription. No PCR products were seen in samples when RT was omitted (-). A 50 bp DNA ladder was used as a size marker (M). GAPDH refers to the internal control gene, glyceraldehyde 3-phosphate dehyrodrogenase.