Figure 1. Expression of GFPuv and mCherry in transformed E. chaffeensis.

Himar1 transposon mutants of E. chaffeensis were generated following electroporation of the constructs into the cell-free organisms recovered from infected ISE6 tick cells and re-cultured in macrophage and tick cells. A drop of culture suspension under a cover slip was imaged under uv-illumination using an Olympus BX61 spinning disk confocal microscope and a Qfire digital camera. Panels A and B represent E. chaffeensis Arkansas isolate expressing mCherry and GFPuv, respectively. Bars = 10 µm.