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. 2013 Feb 14;9(2):e1003171. doi: 10.1371/journal.ppat.1003171

Figure 4. Validation of transposon insertions in the E. chaffeensis genome.

Figure 4

The insertion sites in the E. chaffeensis genome were verified by PCR with primers designed to bind to the genomic region upstream of the insertion sites (forward primer) and to the inserted DNA (spectinomycin resistance gene) (reverse primer). Product sizes for all 9 insertions are different and the predicted size amplicons were observed only in PCRs containing the mutant genomic DNAs as the templates. Lanes 1–9 represent different insertion targets (as listed in Figure 3) amplified from the mutant genomic DNAs of mCherry mutants (lanes 1–5), and GFPuv culture DNAs from the first (lanes 6–8) and second (lane 9) transformation experiments; N, no template control; D, wild type E. chaffeensis DNA used as the template; M, 1 kb+ DNA molecular weight marker.