|
Strains
|
|
|
| DH5α |
E. coli F- φ80lacZΔM15 Δ(lacZYA-argF) U169endA1recA1hsdR17deoRthi-1supE441−
gyrA96relA1 |
Laboratory collection |
| LT2 |
Salmonella Typhimurium LT2 wild-type |
[46]
|
| LT2K7 |
LT2 dgoT::mudK |
This study |
| LT2 ΔdgoR
|
LT2 ΔdgoR
|
This study |
| LT2K7 ΔdgoR
|
LT2 dgoT::mudKΔdgoR
|
This study |
| LT2[P22] |
LT2 P22 lysogen |
This study |
| LT2[P22 Δpid::yfp] |
LT2 P22 Δpid::yfp lysogen |
This study |
| LT2[P22 pid-strep] |
LT2 P22 pid-strep lysogen |
This study |
|
Phages
|
|
|
| P22 wt |
Wild-type P22 phage |
SGSCa
|
| P22 c2
|
Clear mutant of P22 affected in C2 repressor |
SGSCa
|
| P22 H5 |
Virulent derivative of P22 |
Kelly Hughes (University of Utah, USA) |
| P22 HT105/1 int-201
|
Integration deficient mutant of P22 used for generalized transduction |
Kelly Hughes (University of Utah, USA) |
| P22 Δpid::yfp
|
pid replaced by yfp
|
This study |
| P22 Δint Δpid::yfp
|
pid replaced by yfp, int deletion |
This study |
| P22 c2 Δpid::yfp
|
pid replaced by yfp, truncated C2 repressor |
This study |
| P22 pid-strep
|
C-terminal fusion of strep-tag to pid
|
This study |
| P22 c2 pid-strep
|
C-terminal fusion of strep-tag to pid, truncated C2 repressor |
This study |
| P22 Δpid-yfp parS-cat
|
pid replaced by yfp, parS-cat inserted between gtrC and 9
|
This study |
| ES18 |
|
[47]
|
|
Plasmids
|
|
|
| pFPV25 |
Encodes promoterless gfp
|
[48]
|
| pFPV-PBAD-gfp
|
Encodes GFP under control of an arabinose-inducible promoter |
[31]
|
| pFPV-PBAD -pid
|
Encodes Pid under control of an arabinose-inducible promoter |
This study |
| pFPV-PBAD-pidFS
|
As in pFPV-PBAD-pid but harboring a frame shift in the start codon of pid
|
This study |
| pFPV-dgoR
|
Encodes DgoR under its native promoter |
This study |
| pAc |
yfp-frt-cat-frt template for recombineering of yfp
|
[37]
|
| pCP20 |
Encodes Flp for recombining frt sites |
[36]
|
| pKD46 |
Encodes Lambda red genes under control of arabinose inducible promoter |
[35]
|
| pKD3 |
Harbors frt-cat-frt site for construction of deletions by recombineering |
[35]
|
| pALA2705 |
Encodes GFP-ParB under control of the lac promoter |
[41]
|
| pRSet-B-mCherry
|
Used as template for PCR amplification of mCherry
|
Roger Tsien, (University of California, USA) |
| pCW-mcherry-parB
|
Encodes mCherry-ParB under control of the lac promoter. Derived from pALA2705 |
This study |
| pGBKD3-parS
|
Harbors parS-frt-cat-frt site for insertion of the parS site |
[38]
|
