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Live cell imaging of Bud27-Gfp (centromeric plasmid pCM189) in Δbud27 mutant cells at 30°C (A) and Xpo-1 mutant cells for 1 h at 37°C (B). C) Live cell imaging of Bud27ΔNES-GFP in Δbud27 mutant cells at 30°C, showing nuclear localization. D) Rpb8-ECFP (C-terminal ECFP tagged Rpb8) and Bud27ΔNES-GFP in wild-type or Δbud27 mutant cells treated with benomyl (60 µg/ml) at 30°C.
