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A) Growth of wild-type and Δbud27 mutant strains transformed with vectors overexpressing RPB5, at different temperatures or in the presence of rapamycin. pCM and pFL correspond to the control empty vectors. B) Live cell imaging of Rpb8-ECFP in wild-type and Δbud27 mutant cells at 30°C, containing empty vector (pFL) or overexpressing RPB5 (pFL-RPB5).
