Table 2. Plasmids.
Name | Yeast markers | Origin |
pCM189 | ORI (CEN) URA3 | [25] |
pCM189-BUD27 | ORI (CEN) URA3 | This work |
pCM189-BUD27-GFP | ORI (CEN) URA3 | This work |
pCM189-BUD27ΔNES -GFP | ORI (CEN) URA3 | This work |
pCM189-BUD27-TAP | ORI (CEN) URA3 | This work |
pCM189-BUD27ΔPFD-TAP | ORI (CEN) URA3 | This work |
pCM189-BUD27ΔR5-TAP | ORI (CEN) URA3 | This work |
pCM189-BUD27ΔPFDR5-TAP | ORI (CEN) URA3 | This work |
pFL44L | ORI (2 µm) URA3 | [56] |
pFL44L-RPB5 | ORI (2 µm) URA3 | [57] |
pCM189-IWR1ΔNES | ORI (CEN) LEU2 | Gómez-Navarro et al, submitted |
pKT210 | [48] |