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In gastric carcinoma, representative immunostaining for phosphorylation indicators: (A) AKT, (B) pAKT, (C) ERK, (D) pERK; proliferation, growth and apoptosis indicators: (E) Bcl-2, (F) Bax, (G) Ki-67, (H) Cyclin D1, (I) EGFR; angiogenesis indicators: (J) VEGF, (K) CD34 to calculate microvessel density; invasion and metastasis indicators: (L) MMP-2, (M) MMP-9, (N) TIMP-2, (O) E-cadherin. Original magnification, x200. IgG staining was used as a negative control.
