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. 2012 Dec 5;5(2):663–668. doi: 10.3892/ol.2012.1062

Figure 1.

Figure 1.

Mini-array of multiple TAAs with four representative breast cancer sera using slot blot analysis. Each blot represents a duplicate test for autoanti-bodies against a panel of eleven recombinant TAAs, with PBS as a negative control. Purified recombinant protein (100 ng per well) was applied directly to the nitrocellulose membrane using a vacuum device. Membranes were used for the simultaneous detection of autoantibodies in an individual patient’s serum to any of the eleven TAAs, following standard immunoblotting procedures. 1, PBS; 2, survivin; 3, p53; 4, p16; 5, cyclin B1; 6, cyclin D1; 7, cyclin E; 8, Koc; 9, Imp1; 10, p62; 11, CDK2; 12, c-myc. (A) Normal human serum showing no reactivity to any of the eleven TAAs. (B–E) Four representative breast cancer sera showing different antibody profiles with the 11 TAAs. TAA, tumor-associated antigen; PBS, phosphate-buffered saline.