Table 2.
Proteomics analysis of plants in response to pathogenic/symbiotic bacteria.
| Studied organism | Pathogen | Proteomic approach | No. of IP1 | Identified proteins | References |
|---|---|---|---|---|---|
| A. thaliana | P. syringae | 2-DE LC MS/MS | 52 | Abundant proteins (defense-related, Transcription, elongation factor, peroxiredoxin, arginase, lipase/acylhydrolase, PS11, sedoheptulose bisphosphatase, Protein folding and turnover, cytochrome, Cellular transport and ion homeostasis, decreased proteins, metabolism, and proteasome. | Jones et al., 2006a |
| A. thaliana | P. syringae | iTRAQ | 5 | Phosphoproteome changes 4 proteins (dehydrin, co-chaperone, heat shock protein, plastid-associated protein) and ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (RuBisCO LSU). | Jones et al., 2006b |
| A. thaliana | P. syringae | 2-DE; LC MS/MS | 2 | Glutathione S-transferase (GST), peroxiredoxin. | Jones et al., 2004 |
| O. sativa transgenic and/or inoculated with Xanthomonas oryzae pv. oryzae | X. oryzae pv. oryzae | 2-DE MS/MS Protein sequencer | 10 | Accumulation of pathogenesis-related protein (PR) -5, superoxide dismutase, peroxiredoxin, glycine cleavage H protein, glyceraldehydes 3-phosphate dehydrogenase, triose phosphate isomerase, oxygen evolving complex is downregulated. | Mahmood et al., 2006 |
| O. sativa | X. oryzae pv. oryzae | 2-DE, MS/MS | 20 | Defense-related plasma membrane proteins with increased abundance are ATPase, phosphatase, hypersensitive response, prohibitin, zinc finger and C2 domain protein, universal stress protein, heat shock protein, ascorbate peroxidase (APX), alcohol dehydrogenase, and quinone reductase. | Chen et al., 2007 |
| O. sativa | X. campestris pv. Oryzicola | 2-DE, MALDI-TOF MS | 32 | PR-1, PR-10, receptor-type protein kinase, ascorbate peroxidase, adenine triphosphate (ATP) synthase, RuBisCO LSU, ribonuclease, phospholipase, and GTP binding protein. | Li et al., 2012 |
| L. hirsutum (leaflets) | Cl. michiganensis ssp. michiganensis | 2-DE, ESI-MS/MS | 47 | Regulatory proteins accumulated: defense and stress related (PR-3, GST, APX, superoxide dismutase), regulatory proteins, protein synthesis and processing (chaperonin, elongation factor-thermo unstable), carbon metabolism: RuBisCO (LSU, small subunit, activase, epimerase, triose phosphate isomerase), metabolism [glycine cleavage system, oxygen evolving enhancer (OEE)], and ATP production [nucleotide diphosphate kinase (NDK), ATP synthase]. | Coaker et al., 2004 |
| M. truncatula, root | S. meliloti | 2-DE, PMF | 99 | Proteins increased in abundance: defense and stress related (PR-9, -10, APX, superoxide dismutase), S-adenosyl methionine synthase, GST, elongation factor, NDK, protein disulfide isomerase (PDI), OEE, protein synthesis and degradation, isoflavone reductase, hormone dependent proteins, and metabolic proteins (ATP synthase, fructose bisphosphate aldolase). | Mathesius et al., 2003 |
| S. lycopersicum | P. solanacearum | Protein sequencer | 15 | Proteins highly accumulated: protein destination and storage (60 kDa chaperonin, PDI, heat shock protein), protein synthesis, metabolism (RuBisCO activase, plastocyanin, glycine dehydrogenase, OEE), and defense (calgranulin, AMA). | Afroz et al., 2009 |
| Rice (var. Co43) | P. fluorescens KH- 1 (PGPR) | 2-DE, MS, LC MS/MS | 23 | Highly abundant proteins of energy metabolism, photosynthesis, protein degradation and antioxidation: GST, NDK, chaperone, thioredoxin, RuBisCO LSU, and proteosome | Kandasamy et al., 2009 |
| Malus Domestica cv. Holsteiner Cox | P. fluorescens | SDS-PAGE, MS/MS | 5 | PR-2, -3, -4b, -5, -10, ribonuclease-like, endochitinase class III. | Kurkcuoglu et al., 2004 |
| S. lycopersicum | P. solanacearum | Protein sequencer | 15 | Proteins with increased accumulation in response to JA and SA: defense (S-adenosyl methionine synthase, arginase, peroxiredoxin, threonine diaminase, polyphenol oxidase, leucine aminopeptidase), protein synthesis (PDI), protein destination and storage (60 kDa chaperonin), energy (glycine cleavage, ATP synthase), and RuBisCO LSU downregulated. | Afroz et al., 2009 |
| G. max | B. japonicum (symbiotic root nodules) | GC MS, LC-QTOF-MS | 166 | Genes highly expressed: fatty acid, amino acid, carboxylic acid metabolism, disaccharides, isoflavonoids, and glucosinolate. | Brechenmacher et al., 2010 |
1
IP: Number of identified protein.
Abbreviations: ATP, adenine triphosphate; GST, glutathione S-transferase; RuBisCO, ribulose-1, 5-bisphosphate carboxylase/oxygenase; LSU, large subunit; APX, ascorbate peroxidase; PR, pathogenesis-related protein; OEE, oxygen evolving enhancer; PDI, protein disulfide isomerase; NDK, nucleotide diphosphate kinase; 2-DE, two-dimensional polyacrylamide gel electrophoresis; MS, Mass spectrometry; MS/MS, Tandom Mass spectrometry; LC, liquid chromatography; GC, gas chromatography; MALDI-TOF, Matrix-assisted laser desorption/ionization time of flight.