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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Jan 3;33(3):481–488. doi: 10.1161/ATVBAHA.112.255737

Figure 6. Effect of mdivi-1 on platelet accumulation and fibrin generation during thrombus formation following laser injury of cremaster arterioles.

Figure 6

Mouse platelet-specific anti-CD42b antibody conjugated to Dylight 649 (0.1 μg/g body weight) and fibrin-specific mouse anti-human fibrin II β-chain monoclonal antibody conjugated to Alexa 488 (0.5 μg/g body weight) were infused into the mouse. Mdivi-1 (50 mg/kg) was subsequently infused intravenously 5 minutes prior to the initial laser injury. Representative binarized images of the appearance of fluorescence signals associated with platelets (red) and fibrin (green) over 180 seconds following laser-induced vessel wall injury in a wild type mouse are shown in panels (A) vehicle alone and (B) 50 mg/kg mdivi-1. (C) Median integrated platelet fluorescence intensity and (D) median integrated fibrin fluorescence intensity at the injury site are plotted versus time. Data are from 28 thrombi in 3 mice for control condition and 24 thrombi in 3 mice for mdivi-1 condition.