Figure 4.
Time course of cell-associated and cell-free virus production. Growth analysis of (A) TPMV in TBF cells, (B) MVvac2(GFP)N and MVvac2(FΔ32/mvcyt-HΔ90)GFP in Vero-αHis cells. All viruses were inoculated at an MOI of ~0.03. Titers were determined by collection of cellular (circles) and supernatant (squares) fractions every 12 hours over a 60 hour time period for TPMV and MV (filled symbols), and every 24 hours over a 120-hour time period for the hybrid virus (open symbols). Vertical axis: titer by TCID50/ml, horizontal axis: time post-infection.