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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Cancer Gene Ther. 2013 Jan 11;20(2):109–116. doi: 10.1038/cgt.2012.92

Figure 6.

Figure 6

Wild-type and truncated TPMV H protein transport kinetics. CHO-K1 cells were transfected with wild-type F and (A) HHis-tag or (B) H 90 or an empty vector (mock) for 24 hours before being starved for 30 minutes and pulsed with 100μCi/ml 35S labeled methionine and cysteine for 1 hour and then chased for up to 4 hours. After the indicated chase period, cell lysates were collected and H was immunoprecipitated and digested without or with EndoH (−/+ lanes, respectively) followed by fractioning on 7.5% SDS-PAGE. (C) Kinetics of complex oligosaccharide acquisition. Vertical axis: percent EndoH resistance of three experiments for wild type H (diamonds) and HΔ90 (squares). Horizontal axis: time of chase (hours).