Fig. 1.

p38 MAPK subtype expression in human CF. (A) Relative mRNA levels for p38 subtypes in CF from 8 different patients quantified using real-time RT-PCR. Data are expressed as percentage of GAPDH mRNA levels (2^−ΔCT × 100) and depicted as a box and whisker plot (box defines 25th–75th interquartile range, whiskers depict full range, and horizontal line is median). (B) Whole cell homogenates of CF (5 patients) or human saphenous vein endothelial cells (2 patients) were immunoblotted for expression of p38 subtypes. Blot reprobed with β-actin antibody to confirm equal loading.