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. 2013 Jan 4;430(1):419–424. doi: 10.1016/j.bbrc.2012.11.071

Fig. 4.

Fig. 4

Effect of p38α gene silencing on IL-6 and MMP-3 expression. CF were mock-transfected (open bars) or transfected with p38α siRNA (filled bars), cultured for 4 days to promote p38α protein silencing, then stimulated with 10 ng/ml IL-1α for 6–24 h. (A) Real-time RT-PCR data for mRNA levels of IL-6, MMP-3 and p38α, expressed as percentage GAPDH mRNA levels. ∗∗∗P < 0.001, ∗P < 0.05 for effect of gene silencing (6 h, n = 6). (B) ELISA analysis of IL-6 (6 h, n = 5) and MMP-3 (24 h, n = 4) protein levels in conditioned media from IL-1α treated cells. ∗∗P < 0.01 for effect of gene silencing.