Table 1.
Table of primers used for production of feline cDNA and cloning of feline CSF-1R. A kozak sequence (highlighted in bold) was included in CSF-1R forward primer for optimal translation initiation. Feline HRPT primers from Penning et al. [45].
| Primer name | Primer sequence 5′–3′ | Tm (°C) | Size (bp) |
|---|---|---|---|
| Feline HPRT Forward | ACTGTAATGACCAGTCAACAGGGG | 60.0 | 210 |
| Feline HPRT Reverse | TGTATCCAACACTTCGAGGAGTCC | 60.0 | – |
| Feline CSF-1R Forward | GCC ATG GGC CCA AGG GCT | 61.5 | 2946 |
| Feline CSF-1R Reverse | GCA GAA CTG GTA GTT GTT GGG CTG | 61.9 | – |