Figure 5.
Combined kinetics of both undifferentiated, CD45+Col1+CXCR4+ fibrocytes in tracheal allografts, as determined by flow cytometry, and tracheal tissue collagen deposition, as determined by Direct Red 80 densitometry of tracheal allograft sections. Kinetics of control, Goat IgG F(ab′)2 treated animals (A) show a more gradual and less complete decrease in tracheal allograft undifferentiated fibrocyte number with a concomitant more rapid and greater increase in tracheal collagen deposition as compared to anti-CXCL12 F(ab′)2 treated animals (B). Kruskal-Wallis analysis demonstrates that both control and anti-CXCL12 fibrocyte populations (p<0.001) and tracheal collagen deposition (p<0.001) are significantly different.