Table 1.
Candidate genes involved in alkane degradation identified in the B. bassiana EST dataset.
| Gene (accession No.) | Putative function | Gene knockout available? | Phenotype | References |
|---|---|---|---|---|
| CYP52(X1) (GU566074) | Lipid oxidation | Yes | Decreased virulence in insect topical assays/no effect in intrahemoceol injection assays | Zhang et al., 2012 |
| CYP655(C1) (AM409327) | Lipid oxidation | Yes | No phenotype detected thus far | This study |
| CYP5337(A1) (GU566075) | Lipid oxidation | Yes | No phenotype detected thus far | This study |
| CYP52(G11) (GU566076) | Lipid oxidation | Yes | No phenotype detected thus far | This study |
| CYP539(B5) (GU566077) | Lipid oxidation | No | – | – |
| CYP617(N1) (GU566078) | Lipid oxidation | Yes | No phenotype detected thus far | This study |
| CYP53(A26) (GU566079) | Lipid oxidation | Yes | No phenotype detected thus far | This study |
| CYP584(Q1) (GU566080) | Lipid oxidation | No | – | – |
| catA (spore-specific) | Yes | Decreased virulence, thermotolerance and UV resistance. Not tested for alkane degradation | Wang et al., 2012 | |
| catB (secreted) | H2O2 scavenging | Yes | Not tested for alkane degradation | Wang et al., 2012 |
| catP (peroxisomal) | β-oxidation pathway | Yes | Decreased virulence. Not tested for alkane degradation | Wang et al., 2012 |
| catC (cytoplasmic) | H2O2 scavenging | Yes | Not tested for alkane degradation | Wang et al., 2012 |
| catD (secreted peroxidase/catalase) | H2O2 scavenging | Yes | Decreased virulence and UV resistance. Not tested for alkane degradation | Wang et al., 2012 |
| Acyl CoA oxidase | β-oxidation pathway | No | N/A | N/A |
| 3-oxoacyl carrier protein reductase | Biosurfactants synthesis/transport | No | N/A | N/A |
| ADH-2 FALDH | Long-chain alcohol and aldehyde dehydrogenase | No | N/A | N/A |