Figure 5.

Sialidase treatment improves the capacity of monocyte‐derived dendritic cells (MDDCs) to phagocytose pathogenic Escherichia coli isolates. MDDCs were sialidase‐treated or left untreated and incubated for 1 hr, at 4° or 37°, with pathogenic E. coli isolates (I, II, III, IV). The phagocytic capacity was evaluated by flow cytometry as the MFI and values obtained at 4° were subtracted. Values represent the means of at least 10 independent assays. Statistical significance (**P < 0·001 or ***P < 0·0001) refers to the difference between sialidase‐treated and untreated MDDCs.