Figure 7.
Expression of interleukin‐2 (IL‐2) can result in long‐term tumour control and dormancy. 1 × 106 B16/IL‐2.4 tumour cells were injected and the injection site was analysed at various time‐points. (a) Fourteen days following injection, B16/IL‐2.4 cells can still be seen at the tumour site as well as portions of tissue that were excised (arrows indicated melanin‐expressing tumour cells). (b) The injection site was excised 28 days after tumour injection and stained simultaneously for blood vessels using anti‐CD31 (green) and immune cells (anti‐CD45, red), and examined by whole mount histology. White measurement bar indicates 100 μm. (c) Higher magnification of B16/IL‐2.4 tumours at day 28 showing the tumours growing along side vessels. White measurement bar indicates 50 μm. (d) Haematoxylin & eosin staining of a paraffin section of the thigh muscle 100 days following injection of B16/IL‐2.4. Melanin‐expressing tumour cells (black) can be seen between muscle fibres. White measurement bar indicates 100 μm.