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Experimental setup. Blood samples of 63 patients with type 1 diabetes (T1D) were collected and the peripheral blood mononuclear cells (PBMCs) were isolated and analysed by flow cytometry. For the longitudinal study second and third blood samples were collected with a mean interval of 6 and 12 months, respectively. Additionally, DNA isolated from granulocytes of the individuals was used for genotyping for T1D and vitamin D (VD) relevant genes (table 4.01).
